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KMID : 0380620170490010044
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Korean Journal of Food Science and Technology
2017 Volume.49 No. 1 p.44 ~ p.49
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Antioxidant activities of phenolic compounds from Angelica gigas Nakai extract using subcritical water
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Ko Min-Jung
Lee Jeong-Hyun
Nam Hwa-Hyun
Chung Myong-Soo
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Abstract
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Subcritical water extraction can be used to selectively extraction compounds by varying the temperature-dependent dielectric constant of water. This study investigated subcritical water extraction of decursin and nodakenin yields from Angelica gigas Nakai (AN) quantitatively and qualitatively by HPLC, and HPLC-ESI/MS. Total phenolics, total flavonoid contents, and antioxidant activity were determined by DPPH and ABTS radical scavenging activity, including the effects of varying the extraction conditions of temperature (110-200°C) and time (1-20 min) under high pressure (10MPa). By subcritical water extraction under operating conditions of 120-130°C, the maximum yields of decursin (6.64±0.42% in the dried material) and nodakenin (3.71±0.28% in the dried material) were obtained. From 190-200°C the maximum yields of total phenolics (75.97±1.64 mg gallic acid equivalent/g AN), flavonoids (8.56±1.10 mg quercetin equivalent/g AN), DPPH (63.07±1.71%), and ABTS (72.32±2.82%) were obtained.
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KEYWORD
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subcritical water extraction, Angelica gigas Nakai, decursin, nodakenin, antioxidant activity
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